Cohnella lupini sp. nov., an endophytic bacterium isolated from root nodules of Lupinus albus.
Int J Syst Evol Microbiol
; 64(Pt 1): 83-87, 2014 Jan.
Article
in En
| MEDLINE
| ID: mdl-24021729
ABSTRACT
A bacterial strain designated RLAHU4B(T) was isolated from root nodules of Lupinus albus in León (Spain). The 16S rRNA gene sequence of this strain showed similarities lower than 97â% with respect to species of the genus Cohnella. The strain was a Gram-variable, sporulating rod, motile by means of peritrichous flagella, and facultatively anaerobic. It was positive for oxidase, catalase and ß-galactosidase production but negative for urease, amylase and gelatinase. Strain RLAHU4B(T) grew in the presence of 5â% NaCl. MK-7 was the predominant menaquinone and meso-diaminopimelic acid was present in the peptidoglycan. anteiso-C15ââ0, iso-C16ââ0, iso-C15ââ0 and C16ââ0 were the major fatty acids. Major polar lipids of strain RLAHU4B(T) were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, three unknown phospholipids, two unknown aminophospholipids and one unknown lipid. The DNA G+C content was 57.8 mol%. Strain RLAHU4B(T) presented phenotypic differences from all recognized species of the genus Cohnella. The phylogenetic, chemotaxonomic and phenotypic data indicated that strain RLAHU4B(T) belongs to a novel species of the genus Cohnella, for which the name Cohnella lupini sp. nov. is proposed, with strain RLAHU4B(T) (â=âLMG 27416(T)â=âCECT 8236(T)) as the type strain.
Full text:
1
Database:
MEDLINE
Main subject:
Phylogeny
/
Lupinus
/
Root Nodules, Plant
/
Bacillales
Country/Region as subject:
Europa
Language:
En
Year:
2014
Type:
Article