PrimPol, an archaic primase/polymerase operating in human cells.
Mol Cell
; 52(4): 541-53, 2013 Nov 21.
Article
in En
| MEDLINE
| ID: mdl-24207056
ABSTRACT
We describe a second primase in human cells, PrimPol, which has the ability to start DNA chains with deoxynucleotides unlike regular primases, which use exclusively ribonucleotides. Moreover, PrimPol is also a DNA polymerase tailored to bypass the most common oxidative lesions in DNA, such as abasic sites and 8-oxoguanine. Subcellular fractionation and immunodetection studies indicated that PrimPol is present in both nuclear and mitochondrial DNA compartments. PrimPol activity is detectable in mitochondrial lysates from human and mouse cells but is absent from mitochondria derived from PRIMPOL knockout mice. PRIMPOL gene silencing or ablation in human and mouse cells impaired mitochondrial DNA replication. On the basis of the synergy observed with replicative DNA polymerases Polγ and Polε, PrimPol is proposed to facilitate replication fork progression by acting as a translesion DNA polymerase or as a specific DNA primase reinitiating downstream of lesions that block synthesis during both mitochondrial and nuclear DNA replication.
Full text:
1
Database:
MEDLINE
Main subject:
DNA Primase
/
DNA-Directed DNA Polymerase
/
DNA Replication
/
Multifunctional Enzymes
Limits:
Animals
/
Humans
Language:
En
Year:
2013
Type:
Article