Carotenoid cleavage dioxygenase4 is a negative regulator of ß-carotene content in Arabidopsis seeds.
Plant Cell
; 25(12): 4812-26, 2013 Dec.
Article
in En
| MEDLINE
| ID: mdl-24368792
ABSTRACT
Experimental approaches targeting carotenoid biosynthetic enzymes have successfully increased the seed ß-carotene content of crops. However, linkage analysis of seed carotenoids in Arabidopsis thaliana recombinant inbred populations showed that only 21% of quantitative trait loci, including those for ß-carotene, encode carotenoid biosynthetic enzymes in their intervals. Thus, numerous loci remain uncharacterized and underutilized in biofortification approaches. Linkage mapping and genome-wide association studies of Arabidopsis seed carotenoids identified CAROTENOID cleavage dioxygenase4 (CCD4) as a major negative regulator of seed carotenoid content, especially ß-carotene. Loss of CCD4 function did not affect carotenoid homeostasis during seed development but greatly reduced carotenoid degradation during seed desiccation, increasing ß-carotene content 8.4-fold relative to the wild type. Allelic complementation of a ccd4 null mutant demonstrated that single-nucleotide polymorphisms and insertions and deletions at the locus affect dry seed carotenoid content, due at least partly to differences in CCD4 expression. CCD4 also plays a major role in carotenoid turnover during dark-induced leaf senescence, with ß-carotene accumulation again most strongly affected in the ccd4 mutant. These results demonstrate that CCD4 plays a major role in ß-carotene degradation in drying seeds and senescing leaves and suggest that CCD4 orthologs would be promising targets for stabilizing and increasing the level of provitamin A carotenoids in seeds of major food crops.
Full text:
1
Database:
MEDLINE
Main subject:
Plant Proteins
/
Arabidopsis
/
Beta Carotene
/
Arabidopsis Proteins
/
Dioxygenases
Type of study:
Prognostic_studies
Language:
En
Year:
2013
Type:
Article