Engineering nicking enzymes that preferentially nick 5-methylcytosine-modified DNA.

Gutjahr, Alice; Xu, Shuang-yong

Gutjahr, Alice; Xu, Shuang-yong.

Affiliation

Gutjahr A; New England Biolabs, Inc., 240 County Road, Ipswich, MA 01938, USA.
Xu SY; New England Biolabs, Inc., 240 County Road, Ipswich, MA 01938, USA xus@neb.com.

Nucleic Acids Res ; 42(9): e77, 2014 May.

Article in En | MEDLINE | ID: mdl-24609382

Subject(s)

5-Methylcytosine/chemistry; Deoxyribonucleases, Type II Site-Specific/genetics; Viral Proteins/genetics; Bacillus Phages/enzymology; Bacillus anthracis/virology; Base Sequence; Catalytic Domain; DNA Breaks, Single-Stranded; DNA Cleavage; DNA, Circular/chemistry; DNA, Circular/genetics; Deoxyribonucleases, Type II Site-Specific/biosynthesis; Deoxyribonucleases, Type II Site-Specific/chemistry; Manganese/chemistry; Molecular Sequence Data; Plasmids/chemistry; Plasmids/genetics; Potassium Chloride/chemistry; Protein Engineering; Recombinant Fusion Proteins/biosynthesis; Recombinant Fusion Proteins/chemistry; Recombinant Fusion Proteins/genetics; Sequence Analysis, DNA; Viral Proteins/biosynthesis; Viral Proteins/chemistry

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Full text: 1 Database: MEDLINE Main subject: Viral Proteins / Deoxyribonucleases, Type II Site-Specific / 5-Methylcytosine Language: En Year: 2014 Type: Article

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Full text: 1 Database: MEDLINE Main subject: Viral Proteins / Deoxyribonucleases, Type II Site-Specific / 5-Methylcytosine Language: En Year: 2014 Type: Article