Antioxidative and antiproliferative evaluation of 2-(phenylselenomethyl)tetrahydrofuran and 2-(phenylselenomethyl)tetrahydropyran.
J BUON
; 19(1): 283-90, 2014.
Article
in En
| MEDLINE
| ID: mdl-24659677
ABSTRACT
PURPOSE:
To determine the antioxidant and antiproliferative influence of 2-(phenylselenomethyl)tetrahydrofuran (1a) and 2-(phenylselenomethyl)tetrahydropyran (2a) on colon cancer cell line HCT-116 and breast cancer cell line MDA-MB-231.METHODS:
Cell viability was monitored in a dose-dependent manner using MTT assay. The concentration of superoxide anion radical (O2 â¢(-)) was determined spectrophotometrically. Spectrophotometric determination of nitrites (NO2 -) was performed by using the Griess method. Determination of total glutathione (GSH) was also performed spectrophotometrically.RESULTS:
HCT-116 cell line was more sensitive to the effects of the investigated substances than MDA-MB-231 cell line. Also, it was noticed that 1a produced greater effect compared to 2a. Moreover, both investigated compounds decreased to a certain degree the oxidative stress by decreasing the O2â¢(-) and thus the peroxynitrite concentration. At the same time, 1a and 2a acted more efficiently in promoting the endogenous antioxidative capacities (increased GSH concentration) providing better self-defence capabilities for cells.CONCLUSION:
Our findings showed that the investigated selenium compounds play an important role in reducing the levels of reactive oxygen species (ROS); therefore, we believe that, as antioxidants, they could prevent the processes arising as a consequence of oxidative stress, including cancer.
Search on Google
Database:
MEDLINE
Main subject:
Organoselenium Compounds
/
Superoxides
/
Oxidative Stress
/
Furans
/
Antioxidants
Limits:
Humans
Language:
En
Year:
2014
Type:
Article