Comparison of 16S rRNA sequencing with biochemical testing for species-level identification of clinical isolates of Neisseria spp.
World J Microbiol Biotechnol
; 30(8): 2181-8, 2014 Aug.
Article
in En
| MEDLINE
| ID: mdl-24671298
ABSTRACT
We aimed to compare accuracy of genus and species level identification of Neisseria spp. using biochemical testing and 16S rRNA sequence analysis. These methods were evaluated using 85 Neisseria spp. clinical isolates initially identified to the genus level by conventional biochemical tests and API NH system (Bio-Mérieux(®)). In 34 % (29/85), more than one possibility was given by 16S rRNA sequence analysis. In 6 % (5/85), one of the possibilities offered by 16S rRNA gene sequencing, agreed with the result given by biochemical testing. In 4 % (3/85), the same species was given by both methods. 16S rRNA gene sequencing results did not correlate well with biochemical tests.
Full text:
1
Database:
MEDLINE
Main subject:
Bacterial Typing Techniques
/
Gram-Negative Bacterial Infections
/
Neisseria
Type of study:
Diagnostic_studies
/
Prognostic_studies
Language:
En
Year:
2014
Type:
Article