Cellulomonas pakistanensis sp. nov., a moderately halotolerant Actinobacteria.
Int J Syst Evol Microbiol
; 64(Pt 7): 2305-2311, 2014 Jul.
Article
in En
| MEDLINE
| ID: mdl-24733176
ABSTRACT
A rod-shaped, motile, facultatively anaerobic and moderately halotolerant plant-growth-promoting actinobacterial strain, designated NCCP-11(T), was isolated from paddy grains. To delineate its taxonomic position, the strain was subjected to a polyphasic characterization. Cells of strain NCCP-11(T) grew at 10-37 °C (optimum 28-32 °C), at pH 6-9 (optimum pH 7) and in 0-12% (w/v) NaCl (optimum 1-2%) in broth medium. Based on 16S rRNA gene sequence analysis, strain NCCP-11(T) showed highest similarity to the type strains of Cellulomonas hominis (98.99%) and Cellulomonas denverensis (98.09â%) and less than 97 % with other closely related taxa. The chemotaxonomic data [major menaquinone MK-9(H4); cell-wall peptidoglycan type A4ß; major fatty acids anteiso-C15 0, C16 0, C14 0 and anteiso-C17 0; major polar lipids diphosphatidylglycerol, phosphatidylinositol, phosphatidylinositolmannosides and two unknown polar lipids] also supported the affiliation of strain NCCP-11(T) to the genus Cellulomonas. The level of DNA-DNA relatedness between strain NCCP-11(T) and the two type strains mentioned above was less than 42.7%. On the basis of DNA-DNA relatedness, physiological and biochemical characteristics and phylogenetic position, strain NCCP-11(T) can be differentiated from species of the genus Cellulomonas with validly published names and thus represents a novel species, for which the name Cellulomonas pakistanensis sp. nov. is proposed. The type strain is NCCP-11(T) (â= DSM 24792(T)â= JCM 18755(T)â= KCTC 19798(T)).
Full text:
1
Database:
MEDLINE
Main subject:
Phylogeny
/
Oryza
/
Cellulomonas
Country/Region as subject:
Asia
Language:
En
Year:
2014
Type:
Article