Methods for multiplex template sampling in digital PCR assays.
PLoS One
; 9(5): e98341, 2014.
Article
in En
| MEDLINE
| ID: mdl-24854517
ABSTRACT
The efficient use of digital PCR (dPCR) for precision copy number analysis requires high concentrations of target molecules that may be difficult or impossible to obtain from clinical samples. To solve this problem we present a strategy, called Multiplex Template Sampling (MTS), that effectively increases template concentrations by detecting multiple regions of fragmented target molecules. Three alternative assay approaches are presented for implementing MTS analysis of chromosome 21, providing a 10-fold concentration enhancement while preserving assay precision.
Full text:
1
Database:
MEDLINE
Main subject:
Multiplex Polymerase Chain Reaction
Limits:
Humans
Language:
En
Year:
2014
Type:
Article