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Proliferation and enrichment of CD133(+) glioblastoma cancer stem cells on 3D chitosan-alginate scaffolds.
Kievit, Forrest M; Florczyk, Stephen J; Leung, Matthew C; Wang, Kui; Wu, Jennifer D; Silber, John R; Ellenbogen, Richard G; Lee, Jerry S H; Zhang, Miqin.
Affiliation
  • Kievit FM; Department of Neurological Surgery, University of Washington, Seattle, WA 98195, USA.
  • Florczyk SJ; Department of Materials Science & Engineering, University of Washington, Seattle, WA 98195, USA.
  • Leung MC; Department of Materials Science & Engineering, University of Washington, Seattle, WA 98195, USA.
  • Wang K; Department of Materials Science & Engineering, University of Washington, Seattle, WA 98195, USA.
  • Wu JD; Department of Medicine, School of Medicine, University of Washington, Seattle, WA 98195, USA; Department of Microbiology and Immunology, Hollings Cancer Center, Medical University of South Carolina, Charleston, SC 29425, USA.
  • Silber JR; Department of Neurological Surgery, University of Washington, Seattle, WA 98195, USA.
  • Ellenbogen RG; Department of Neurological Surgery, University of Washington, Seattle, WA 98195, USA; Department of Radiology, University of Washington, Seattle, WA 98195, USA.
  • Lee JS; Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD 21218, USA; Center for Strategic Scientific Initiatives, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA.
  • Zhang M; Department of Neurological Surgery, University of Washington, Seattle, WA 98195, USA; Department of Materials Science & Engineering, University of Washington, Seattle, WA 98195, USA. Electronic address: mzhang@u.washington.edu.
Biomaterials ; 35(33): 9137-43, 2014 Nov.
Article in En | MEDLINE | ID: mdl-25109438
ABSTRACT
Emerging evidence implicates cancer stem cells (CSCs) as primary determinants of the clinical behavior of human cancers, representing an ideal target for next-generation anti-cancer therapies. However CSCs are difficult to propagate in vitro, severely limiting the study of CSC biology and drug development. Here we report that growing cells from glioblastoma (GBM) cell lines on three dimensional (3D) porous chitosan-alginate (CA) scaffolds dramatically promotes the proliferation and enrichment of cells possessing the hallmarks of CSCs. CA scaffold-grown cells were found more tumorigenic in nude mouse xenografts than cells grown from monolayers. Growing in CA scaffolds rapidly promoted expression of genes involved in the epithelial-to-mesenchymal transition that has been implicated in the genesis of CSCs. Our results indicate that CA scaffolds have utility as a simple and inexpensive means to cultivate CSCs in vitro in support of studies to understand CSC biology and develop more effective anti-cancer therapies.
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Full text: 1 Database: MEDLINE Main subject: Neoplastic Stem Cells / Chitosan / Cell Proliferation / Alginates / Tissue Scaffolds Limits: Animals / Humans Language: En Year: 2014 Type: Article
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Full text: 1 Database: MEDLINE Main subject: Neoplastic Stem Cells / Chitosan / Cell Proliferation / Alginates / Tissue Scaffolds Limits: Animals / Humans Language: En Year: 2014 Type: Article