Integrated approach using multistep enzyme digestion, TiO2 enrichment, and database search for in-depth phosphoproteomic profiling.

Han, Dohyun; Jin, Jonghwa; Yu, Jiyoung; Kim, Kyunggon; Kim, Youngsoo

Han, Dohyun; Jin, Jonghwa; Yu, Jiyoung; Kim, Kyunggon; Kim, Youngsoo.

Affiliation

Han D; Departments of Biomedical Sciences, Medical Research Center, Seoul National University College of Medicine, Seoul, Korea; Biomedical Engineering, Medical Research Center, Seoul National University College of Medicine, Seoul, Korea; Institute of Medical & Biological Engineering, Medical Research Center, Seoul National University College of Medicine, Seoul, Korea.

Proteomics ; 15(2-3): 618-23, 2015 Jan.

Article in En | MEDLINE | ID: mdl-25159016

Subject(s)

Proteome/analysis; Proteomics/methods; Animals; Chromatography, Liquid/methods; Databases, Protein; Humans; Phosphorylation; Protein Processing, Post-Translational; Proteome/isolation & purification; Systems Biology/methods; Tandem Mass Spectrometry/methods; Titanium/chemistry

Key words

LC-MS/MS; Phosphoproteome; Post-translational modification; Proteome profiling; Systems biology

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Full text: 1 Database: MEDLINE Main subject: Proteome / Proteomics Limits: Animals / Humans Language: En Year: 2015 Type: Article

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Full text: 1 Database: MEDLINE Main subject: Proteome / Proteomics Limits: Animals / Humans Language: En Year: 2015 Type: Article