The low-affinity nerve growth factor receptor p75NTR identifies a transient stem cell-like state in oral squamous cell carcinoma cells.
J Oral Pathol Med
; 44(6): 410-9, 2015 Jul.
Article
in En
| MEDLINE
| ID: mdl-25212757
ABSTRACT
BACKGROUND:
Although several markers have been used for enrichment of cells with stem cell-like properties in oral squamous cell carcinoma (OSCC), isolation of a pure subpopulation is still a challenging task. Normal oral and esophageal keratinocyte stem cells have been previously isolated using the low-affinity nerve growth factor receptor p75NTR.OBJECTIVE:
To investigate the potential of p75NTR as a marker for identification and isolation of oral cancer cells with stem cell-like properties.METHODS:
Subpopulations of cells with high or low expression of p75NTR were sorted from OSCC-derived cells and compared for sphere/colony formation, in vivo tumor formation ability, expression of stem cell-related molecules, cell cycle distribution and drug resistance.RESULTS:
p75NTR(High) cells exhibited statistically significant higher stem cell properties than p75NTR(Low) cells in all assays performed. Nevertheless, p75NTR(Low) subpopulation did also exhibit some stem cell features, but to a lesser extent. Propagation of p75NTR(Low) cells for several passages in culture showed that the expression of p75NTR could rise spontaneously. This finding was also supported by the similar expression of p75NTR by the xenografts generated by both subpopulations in NOD\SCID IL2Rg(null) mice.CONCLUSION:
p75NTR can be used for isolating a subpopulation enriched for cells with stem cell-like properties in OSCC. De novo generation of p75NTR(High) cells from p75NTR(Low) cells suggests either that there is another subpopulation with stem cell features within the p75NTR(Low) cells, or that the p75NTR(Low) cells can dedifferentiate due to a contextually regulated equilibrium between stem cell-like cells and transit-amplifying neoplastic progenitors.Key words
Full text:
1
Database:
MEDLINE
Main subject:
Neoplastic Stem Cells
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Mouth Neoplasms
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Carcinoma, Squamous Cell
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Biomarkers, Tumor
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Receptors, Nerve Growth Factor
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Head and Neck Neoplasms
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Nerve Tissue Proteins
Type of study:
Prognostic_studies
Limits:
Animals
/
Humans
Language:
En
Year:
2015
Type:
Article