Proteomic identification of mammalian cell surface derived glycosylphosphatidylinositol-anchored proteins through selective glycan enrichment.

Cortes, Leslie K; Vainauskas, Saulius; Dai, Nan; McClung, Colleen M; Shah, Manesh; Benner, Jack S; Corrêa, Ivan R; VerBerkmoes, Nathan C; Taron, Christopher H

Cortes, Leslie K; Vainauskas, Saulius; Dai, Nan; McClung, Colleen M; Shah, Manesh; Benner, Jack S; Corrêa, Ivan R; VerBerkmoes, Nathan C; Taron, Christopher H.

Affiliation

Cortes LK; New England Biolabs, Inc, Ipswich, MA, USA.

Proteomics ; 14(21-22): 2471-84, 2014 Nov.

Article in En | MEDLINE | ID: mdl-25262930

Subject(s)

Cell Membrane/chemistry; GPI-Linked Proteins/analysis; Polysaccharides/analysis; Proteomics; Alkynes/chemistry; Animals; Cell Line; Chromatography, Liquid; GPI-Linked Proteins/isolation & purification; HeLa Cells; Humans; Polysaccharides/isolation & purification; Proteomics/methods; Tandem Mass Spectrometry

Key words

Alkyne agarose; Azido sugar analog; Glycoproteins; Lectin affinity; Polarized epithelial cells

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Full text: 1 Database: MEDLINE Main subject: Polysaccharides / Cell Membrane / Proteomics / GPI-Linked Proteins Type of study: Diagnostic_studies Limits: Animals / Humans Language: En Year: 2014 Type: Article

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