Evaluation of data-dependent and -independent mass spectrometric workflows for sensitive quantification of proteins and phosphorylation sites.

Bauer, Manuel; Ahrné, Erik; Baron, Anna P; Glatter, Timo; Fava, Luca L; Santamaria, Anna; Nigg, Erich A; Schmidt, Alexander

Bauer, Manuel; Ahrné, Erik; Baron, Anna P; Glatter, Timo; Fava, Luca L; Santamaria, Anna; Nigg, Erich A; Schmidt, Alexander.

Affiliation

Bauer M; Biozentrum, University of Basel , Klingelbergstrasse 50/70, CH-4056 Basel, Switzerland.

J Proteome Res ; 13(12): 5973-88, 2014 Dec 05.

Article in En | MEDLINE | ID: mdl-25330945

Subject(s)

Chromatography, Liquid/methods; Mass Spectrometry/methods; Phosphopeptides/analysis; Tandem Mass Spectrometry/methods; Amino Acid Sequence; Binding Sites; Cell Cycle Proteins/metabolism; Cell Extracts/analysis; HeLa Cells; Humans; Molecular Sequence Data; Nuclear Proteins/metabolism; Phosphopeptides/metabolism; Phosphorylation; Proteomics/methods; Reproducibility of Results

Key words

MAD1; Targeted mass spectrometry; cell cycle; data-dependent acquisition; directed mass spectrometry; limit of detection; peptide quantification; phosphorylation

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Full text: 1 Database: MEDLINE Main subject: Phosphopeptides / Mass Spectrometry / Chromatography, Liquid / Tandem Mass Spectrometry Type of study: Diagnostic_studies / Evaluation_studies / Prognostic_studies Limits: Humans Language: En Year: 2014 Type: Article

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