Catenisphaera adipataccumulans gen. nov., sp. nov., a member of the family Erysipelotrichaceae isolated from an anaerobic digester.
Int J Syst Evol Microbiol
; 65(Pt 3): 805-810, 2015 Mar.
Article
in En
| MEDLINE
| ID: mdl-25481294
ABSTRACT
An obligately anaerobic bacterium, designated strain GK12(T), was isolated from an anaerobic digester in Fukagawa, Hokkaido Prefecture, Japan. The cells of strain GK12(T) were non-motile, non-spore-forming cocci that commonly occurred in chains. 16S rRNA gene sequence analysis revealed that strain GK12(T) was affiliated with the family Erysipelotrichaceae in the phylum Firmicutes and showed 91.8â% sequence similarity to the most closely related species, Faecalicoccus acidiformans. The strain grew at 30-50 °C (optimally at 40 °C) and at pH 5.5-8.5 (optimally at pH 7.5). The main end product of glucose fermentation was lactate. Yeast extract was required for growth. The strain contained C14ââ0, C14ââ0 1,1-dimethoxyalkane (DMA), C16ââ0 DMA and C18ââ0 DMA as the major cellular fatty acids (>10â% of the total). The polar lipid profile was composed of phosphatidylglycerol, phosphatidylinositol and an unidentified phospholipid. The whole-cell sugars were galactose, rhamnose and ribose. The cell-wall murein contained alanine, glutamic acid, lysine, serine and threonine, but not diaminopimelic acid. The G+C content of the genomic DNA was 47.7 mol%. Based on phenotypic, phylogenetic and chemotaxonomic properties, a novel genus and species, Catenisphaera adipataccumulans gen. nov., sp. nov., is proposed to accommodate strain GK12(T) (â=âNBRC 108915(T)â=âDSM 25799(T)).
Full text:
1
Database:
MEDLINE
Main subject:
Phylogeny
/
Bacteria, Anaerobic
/
Gram-Positive Rods
Country/Region as subject:
Asia
Language:
En
Year:
2015
Type:
Article