Dexamethasone Controlled Release on TGF-ß1 Treated Vocal Fold Fibroblasts.
Ann Otol Rhinol Laryngol
; 124(7): 572-8, 2015 Jul.
Article
in En
| MEDLINE
| ID: mdl-25667215
ABSTRACT
OBJECTIVE:
Corticosteroids may be beneficial in treating vocal fold scarring. Current drug delivery methods do not permit controlled corticosteroid release. Here we investigate the effects of poly-lactic-co-glycolic acid (PLGA) microparticles loaded with the corticosteroid dexamethasone in reducing collagen synthesis and inflammation in vocal fold fibroblasts treated with and without TGF-ß1. STUDYDESIGN:
Experimental, in vitro study.METHODS:
PLGA microparticles of differing molecular weight and terminating moieties were synthesized using a hydrogel template method. The release of dexamethasone was characterized from these microparticles over 4 days. Based on the release studies, ester-terminated low molecular weight PLGA microparticles were loaded with dexamethasone and applied to TGF-ß1 treated vocal fold fibroblasts for 4 days. Quantitative polymerase chain reaction (qPCR) and enzyme-linked immunosorbent assays (ELISAs) were used to assess the effects of released dexamethasone on collagen synthesis and inflammatory mediators.RESULTS:
COL3A1 and COL1A2 were significantly down-regulated after exposure to ester-terminated low molecular weight PLGA microparticles loaded with dexamethasone. The loaded microparticles also reduced interleukin-6 synthesis.CONCLUSION:
These data show promise in using a PLGA microparticle-based delivery system to control dexamethasone release over 4 days. Our findings lay the groundwork for developing more effective treatments for vocal fold scarring.Key words
Full text:
1
Database:
MEDLINE
Main subject:
Polyglycolic Acid
/
Vocal Cords
/
Dexamethasone
/
Lactic Acid
/
Transforming Growth Factor beta1
/
Vocal Cord Dysfunction
Limits:
Humans
Language:
En
Year:
2015
Type:
Article