Evaluation of the swab sampling method to recover viruses from fomites.
J Virol Methods
; 217: 24-7, 2015 Jun 01.
Article
in En
| MEDLINE
| ID: mdl-25704597
ABSTRACT
The monitoring of virus contamination on fomites, especially at hospitals has been used for a more effective evaluation of the microbiological quality of surfaces. Swab sampling is the method used currently, although the use of an internal control process (ICP) has not yet been assessed. The aim of this study is to determine the recovery rate of murine norovirus 1 (MNV-1) and bacteriophage PP7 on different surfaces in order to assess their potential use as an ICP. For this purpose both viruses were spiked experimentally both on porous and non-porous formic as well as on rubberized surfaces. Quantitative PCR (qPCR) showed a variable efficiency with a percentage recovery ranging from 0.6 to 77% according to viruses and surfaces. A global analysis suggested that MNV-1 could be used as a potential ICP for the swab sampling method.
Key words
Full text:
1
Database:
MEDLINE
Main subject:
Bacteriophages
/
Virology
/
Norovirus
/
Fomites
Language:
En
Year:
2015
Type:
Article