Hydrogen/deuterium exchange mass spectrometry applied to IL-23 interaction characteristics: potential impact for therapeutics.
Expert Rev Proteomics
; 12(2): 159-69, 2015 Apr.
Article
in En
| MEDLINE
| ID: mdl-25711416
ABSTRACT
IL-23 is an important therapeutic target for the treatment of inflammatory diseases. Adnectins are targeted protein therapeutics that are derived from domain III of human fibronectin and have a similar protein scaffold to antibodies. Adnectin 2 was found to bind to IL-23 and compete with the IL-23/IL-23R interaction, posing a potential protein therapeutic. Hydrogen/deuterium exchange mass spectrometry and computational methods were applied to probe the binding interactions between IL-23 and Adnectin 2 and to determine the correlation between the two orthogonal methods. This review summarizes the current structural knowledge about IL-23 and focuses on the applicability of hydrogen/deuterium exchange mass spectrometry to investigate the higher order structure of proteins, which plays an important role in the discovery of new and improved biotherapeutics.
Key words
Full text:
1
Database:
MEDLINE
Main subject:
Biological Therapy
/
Deuterium
/
Interleukin-23
/
Hydrogen
Limits:
Humans
Language:
En
Year:
2015
Type:
Article