Quantitative determination of pectic substances as an example of a rhamnopolysaccharide assay.
J Biochem Biophys Methods
; 19(2-3): 215-22, 1989.
Article
in En
| MEDLINE
| ID: mdl-2584608
ABSTRACT
A quantitative enzymatic assay for rhamnopolysaccharides is described. The procedure is shown with pectic substances as an example. The test is based on the enzymatic degradation of the macromolecules to liberate L-rhamnose. This sugar can be quantitatively determined with the help of L-rhamnose dehydrogenase under concomitant reduction of NAD, thus allowing the quantitative evaluation of the original pectin.
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Database:
MEDLINE
Main subject:
Polysaccharides
/
Pectins
Language:
En
Year:
1989
Type:
Article