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Cultivation of Heligmosomoides polygyrus: an immunomodulatory nematode parasite and its secreted products.
Johnston, Chris J C; Robertson, Elaine; Harcus, Yvonne; Grainger, John R; Coakley, Gillian; Smyth, Danielle J; McSorley, Henry J; Maizels, Rick.
Affiliation
  • Johnston CJ; Institute of Immunology and Infection Research, University of Edinburgh.
  • Robertson E; Institute of Immunology and Infection Research, University of Edinburgh.
  • Harcus Y; Institute of Immunology and Infection Research, University of Edinburgh.
  • Grainger JR; Manchester Collaborative Centre for Inflammation Research.
  • Coakley G; Institute of Immunology and Infection Research, University of Edinburgh.
  • Smyth DJ; Institute of Immunology and Infection Research, University of Edinburgh.
  • McSorley HJ; Institute of Immunology and Infection Research, University of Edinburgh.
  • Maizels R; Institute of Immunology and Infection Research, University of Edinburgh; rick.maizels@ed.ac.uk.
J Vis Exp ; (98): e52412, 2015 Apr 06.
Article in En | MEDLINE | ID: mdl-25867600
ABSTRACT
Heligmosomoides polygyrus (formerly known as Nematospiroides dubius, and also referred to by some as H. bakeri) is a gastrointestinal helminth that employs multiple immunomodulatory mechanisms to establish chronic infection in mice and closely resembles prevalent human helminth infections. H. polygyrus has been studied extensively in the field of helminth-derived immune regulation and has been found to potently suppress experimental models of allergy and autoimmunity (both with active infection and isolated secreted products). The protocol described in this paper outlines management of the H. polygyrus life cycle for consistent production of L3 larvae, recovery of adult parasites, and collection of their excretory-secretory products (HES).
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Full text: 1 Database: MEDLINE Main subject: Nematospiroides dubius Type of study: Prognostic_studies Limits: Animals Language: En Year: 2015 Type: Article
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PubMed Links
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Full text: 1 Database: MEDLINE Main subject: Nematospiroides dubius Type of study: Prognostic_studies Limits: Animals Language: En Year: 2015 Type: Article