Analysis of mRNA Translation Rate in Mouse Embryonic Stem Cells.

Rahim, Anisa B; Vardy, Leah A

Rahim, Anisa B; Vardy, Leah A.

Affiliation

Rahim AB; Institute of Medical Biology, A*STAR, 8A Biomedical Grove, #06-06 Immunos, Singapore, 138648, Singapore.
Vardy LA; Institute of Medical Biology, A*STAR, 8A Biomedical Grove, #06-06 Immunos, Singapore, 138648, Singapore. leah.vardy@imb.a-star.edu.sg.

Methods Mol Biol ; 1341: 143-55, 2016.

Article in En | MEDLINE | ID: mdl-26084596

Subject(s)

Mouse Embryonic Stem Cells/metabolism; Neural Stem Cells/cytology; Protein Biosynthesis; RNA, Messenger/genetics; Animals; Cell Culture Techniques/methods; Cell Differentiation; Cell Fractionation/methods; Centrifugation, Density Gradient/methods; Mice; Mouse Embryonic Stem Cells/cytology; Neural Stem Cells/metabolism; Polyribosomes/genetics; RNA, Messenger/isolation & purification; Real-Time Polymerase Chain Reaction/methods

Key words

Differentiation; Polysome; Quantitative real-time PCR; Stem cells; Translation

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Full text: 1 Database: MEDLINE Main subject: Protein Biosynthesis / RNA, Messenger / Neural Stem Cells / Mouse Embryonic Stem Cells Limits: Animals Language: En Year: 2016 Type: Article

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