PP011. Placental expression of the major protein components of caveolae, eNOS and iNOS in pre-eclampsia.

ABSTRACT

INTRODUCTION: Caveolins and Cavins are the major protein components of caveolae and regulate many cardiovascular functions. Caveolin-1 inhibits eNOS activity. The possible regulation of vascular reactivity/blood pressure by the caveolae are of interest in relation to pre-eclampsia (PE). We hypothesised that expression of Caveolin/Cavin genes would be reduced, paralleling the up-regulated eNOS in PE compared to normotensive controls (NC). OBJECTIVES: To analyse the placental mRNA expression of Caveolins1-3 and, Cavins1-4, eNOS and iNOS; and protein of caveolin-1, cavin-1 and eNOS in NC and PE placentae from White European women. METHODS: Following ethical approval and informed written consent, placental biopsies were taken midway between the cord and periphery, avoiding infarcts, from 24 NC and 23 PE patients. Gene expression was measured by qRT-PCR. Protein localization was identified by immunohistochemistry and expression semi-quantitatively assessed. RESULTS: Results of mRNA/proteins are shown on table below. Protein expression was localised to the cytotrophoblast and syncytiotrophoblast. No differences were found for any other gene/protein. CONCLUSION: As well as their known effects on eNOS expression, caveolae mediate internalisation of numerous hormone receptors, thus potentially changing pressor and depressor responsiveness. This is the first time that structural determinants of caveolae have been studied in NC and PE pregnancy. FUNDING: Tommy's, CAPES.