Validity of Tissue Microarray by Immunohistochemistry.
Clin Lab
; 61(5-6): 569-74, 2015.
Article
in En
| MEDLINE
| ID: mdl-26118190
ABSTRACT
BACKGROUND:
Tissue microarrays (TMAs) extract designated areas of tissue paraffin blocks in several units that are millimeters in diameter in a cylindrical fashion, array dozens of these tissue specimens, and then re-embed them. Here, a TMA was utilized to analyze renal cell carcinoma (RCC) specimens with anti-FABP7 and anti-Brn2 antibodies.METHODS:
Paraffin-embedded specimens from 114 RCC patients were immunostained with anti-FABP7 and anti-Brn2 antibodies to examine the rate of agreement between the staining of TMA grafts compared to conventional tissue slice grafts. The staining area of the tumor was also examined.RESULTS:
The positive ratio of anti-FABP7 was 74% and of anti-Brn2 was 57%. The rate of agreement of each antibody was 100% regardless of tumor size before extraction.CONCLUSIONS:
Immunostaining of TMA slices might be effective for the analysis of RCC specimens.
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Database:
MEDLINE
Main subject:
Immunohistochemistry
/
Carcinoma, Renal Cell
/
Carrier Proteins
/
Tumor Suppressor Proteins
/
Tissue Array Analysis
/
Kidney Neoplasms
Limits:
Humans
Language:
En
Year:
2015
Type:
Article