PLGA-Based Nanoparticles: a Safe and Suitable Delivery Platform for Osteoarticular Pathologies.
Pharm Res
; 32(12): 3886-98, 2015 Dec.
Article
in En
| MEDLINE
| ID: mdl-26134451
ABSTRACT
PURPOSE:
Despite the promising applications of PLGA based particles, studies examining the fate and consequences of these particles after intra-articular administration in the joint are scanty. This study was carried out to evaluate the neutrality of the unloaded delivery system on different articular cell types. To facilitate tracking, we have thus developed a fluorescent core of particles, combined to a hyaluronate shell for cell recognition.METHODS:
Fluorescence pictures were taken at time intervals to assess the internalization and the corresponding inflammatory response was monitored by RT-qPCR and biochemical measurements. After NPs pre-treatment, mesenchymal stem cells (MSCs) were cultured into chondrogenic, adipogenic or osteogenic differentiation media, to investigate if NPs exposure interferes with differentiation ability. Finally, intra-articular injections were performed in healthy rat knees and joint's structure analysed by histological studies.RESULTS:
Particles were detected in cytoplasm 8 h after exposure. Internalization led to a slight and reversible increase of inflammatory markers, but lower than in inflammatory conditions. We have confirmed particles exposure minimal neutrality on MSCs pluripotency. Histological exams of joint after intra-articular injections do not demonstrate any side effects of NPs.CONCLUSIONS:
Our findings suggest that such a delivery platform is well tolerated locally and could be used to deliver active molecules to the joint.Key words
Full text:
1
Database:
MEDLINE
Main subject:
Polyglycolic Acid
/
Drug Carriers
/
Lactic Acid
/
Nanoparticles
Type of study:
Etiology_studies
Language:
En
Year:
2015
Type:
Article