Identification of a Class of Protein ADP-Ribosylating Sirtuins in Microbial Pathogens.
Mol Cell
; 59(2): 309-20, 2015 Jul 16.
Article
in En
| MEDLINE
| ID: mdl-26166706
ABSTRACT
Sirtuins are an ancient family of NAD(+)-dependent deacylases connected with the regulation of fundamental cellular processes including metabolic homeostasis and genome integrity. We show the existence of a hitherto unrecognized class of sirtuins, found predominantly in microbial pathogens. In contrast to earlier described classes, these sirtuins exhibit robust protein ADP-ribosylation activity. In our model organisms, Staphylococcus aureus and Streptococcus pyogenes, the activity is dependent on prior lipoylation of the target protein and can be reversed by a sirtuin-associated macrodomain protein. Together, our data describe a sirtuin-dependent reversible protein ADP-ribosylation system and establish a crosstalk between lipoylation and mono-ADP-ribosylation. We propose that these posttranslational modifications modulate microbial virulence by regulating the response to host-derived reactive oxygen species.
Full text:
1
Database:
MEDLINE
Main subject:
Bacterial Proteins
/
Adenosine Diphosphate Ribose
/
Sirtuins
Type of study:
Diagnostic_studies
Limits:
Humans
Language:
En
Year:
2015
Type:
Article