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TRIzol and Alu qPCR-based quantification of metastatic seeding within the skeleton.
Preston Campbell, J; Mulcrone, P; Masood, S K; Karolak, M; Merkel, A; Hebron, K; Zijlstra, A; Sterling, J; Elefteriou, F.
Affiliation
  • Preston Campbell J; 1] Department of Pharmacology, Vanderbilt University, Nashville, Tennessee, United States of America [2] Vanderbilt Center for Bone Biology, Vanderbilt University, Nashville, Tennessee, United States of America.
  • Mulcrone P; 1] Department of Pharmacology, Vanderbilt University, Nashville, Tennessee, United States of America [2] Vanderbilt Center for Bone Biology, Vanderbilt University, Nashville, Tennessee, United States of America.
  • Masood SK; Vanderbilt Center for Bone Biology, Vanderbilt University, Nashville, Tennessee, United States of America.
  • Karolak M; 1] Department of Pharmacology, Vanderbilt University, Nashville, Tennessee, United States of America [2] Vanderbilt Center for Bone Biology, Vanderbilt University, Nashville, Tennessee, United States of America.
  • Merkel A; 1] Department of Veterans Affairs (VISN 9), Nashville, Tennessee, United States of America [2] Vanderbilt Center for Bone Biology, Vanderbilt University, Nashville, Tennessee, United States of America.
  • Hebron K; 1] Department of Pathology, Microbiology, and Immunology, Vanderbilt University, Nashville, Tennessee, United States of America [2] Vanderbilt Center for Bone Biology, Vanderbilt University, Nashville, Tennessee, United States of America.
  • Zijlstra A; 1] Department of Pathology, Microbiology, and Immunology, Vanderbilt University, Nashville, Tennessee, United States of America [2] Vanderbilt Center for Bone Biology, Vanderbilt University, Nashville, Tennessee, United States of America.
  • Sterling J; 1] Department of Veterans Affairs (VISN 9), Nashville, Tennessee, United States of America [2] Vanderbilt Center for Bone Biology, Vanderbilt University, Nashville, Tennessee, United States of America.
  • Elefteriou F; 1] Department of Pharmacology, Vanderbilt University, Nashville, Tennessee, United States of America [2] Vanderbilt Center for Bone Biology, Vanderbilt University, Nashville, Tennessee, United States of America.
Sci Rep ; 5: 12635, 2015 Aug 14.
Article in En | MEDLINE | ID: mdl-26271202
ABSTRACT
Current methods for detecting disseminated tumor cells in the skeleton are limited by expense and technical complexity. We describe a simple and inexpensive method to quantify, with single cell sensitivity, human metastatic cancer in the mouse skeleton, concurrently with host gene expression, using TRIzol-based DNA/RNA extraction and Alu sequence qPCR amplification. This approach enables precise quantification of tumor cells and corresponding host gene expression during metastatic colonization in xenograft models.
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Full text: 1 Database: MEDLINE Main subject: Bone Neoplasms / Biomarkers, Tumor / Gene Expression Profiling / Real-Time Polymerase Chain Reaction Type of study: Diagnostic_studies / Prognostic_studies Limits: Animals Language: En Year: 2015 Type: Article
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Full text: 1 Database: MEDLINE Main subject: Bone Neoplasms / Biomarkers, Tumor / Gene Expression Profiling / Real-Time Polymerase Chain Reaction Type of study: Diagnostic_studies / Prognostic_studies Limits: Animals Language: En Year: 2015 Type: Article