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Differential Regulation of Clathrin and Its Adaptor Proteins during Membrane Recruitment for Endocytosis.
Wang, Chao; Hu, Tianwei; Yan, Xu; Meng, Tingting; Wang, Yutong; Wang, Qingmei; Zhang, Xiaoyue; Gu, Ying; Sánchez-Rodríguez, Clara; Gadeyne, Astrid; Lin, Jinxing; Persson, Staffan; Van Damme, Daniël; Li, Chuanyou; Bednarek, Sebastian Y; Pan, Jianwei.
Affiliation
  • Wang C; College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua 321004, China (C.W., T.H., X.Y., T.M., Y.W., Q.W., J.P.);State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China (X.Z., C.L.);Department of B
  • Hu T; College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua 321004, China (C.W., T.H., X.Y., T.M., Y.W., Q.W., J.P.);State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China (X.Z., C.L.);Department of B
  • Yan X; College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua 321004, China (C.W., T.H., X.Y., T.M., Y.W., Q.W., J.P.);State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China (X.Z., C.L.);Department of B
  • Meng T; College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua 321004, China (C.W., T.H., X.Y., T.M., Y.W., Q.W., J.P.);State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China (X.Z., C.L.);Department of B
  • Wang Y; College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua 321004, China (C.W., T.H., X.Y., T.M., Y.W., Q.W., J.P.);State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China (X.Z., C.L.);Department of B
  • Wang Q; College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua 321004, China (C.W., T.H., X.Y., T.M., Y.W., Q.W., J.P.);State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China (X.Z., C.L.);Department of B
  • Zhang X; College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua 321004, China (C.W., T.H., X.Y., T.M., Y.W., Q.W., J.P.);State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China (X.Z., C.L.);Department of B
  • Gu Y; College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua 321004, China (C.W., T.H., X.Y., T.M., Y.W., Q.W., J.P.);State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China (X.Z., C.L.);Department of B
  • Sánchez-Rodríguez C; College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua 321004, China (C.W., T.H., X.Y., T.M., Y.W., Q.W., J.P.);State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China (X.Z., C.L.);Department of B
  • Gadeyne A; College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua 321004, China (C.W., T.H., X.Y., T.M., Y.W., Q.W., J.P.);State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China (X.Z., C.L.);Department of B
  • Lin J; College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua 321004, China (C.W., T.H., X.Y., T.M., Y.W., Q.W., J.P.);State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China (X.Z., C.L.);Department of B
  • Persson S; College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua 321004, China (C.W., T.H., X.Y., T.M., Y.W., Q.W., J.P.);State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China (X.Z., C.L.);Department of B
  • Van Damme D; College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua 321004, China (C.W., T.H., X.Y., T.M., Y.W., Q.W., J.P.);State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China (X.Z., C.L.);Department of B
  • Li C; College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua 321004, China (C.W., T.H., X.Y., T.M., Y.W., Q.W., J.P.);State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China (X.Z., C.L.);Department of B
  • Bednarek SY; College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua 321004, China (C.W., T.H., X.Y., T.M., Y.W., Q.W., J.P.);State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China (X.Z., C.L.);Department of B
  • Pan J; College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua 321004, China (C.W., T.H., X.Y., T.M., Y.W., Q.W., J.P.);State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China (X.Z., C.L.);Department of B
Plant Physiol ; 171(1): 215-29, 2016 05.
Article in En | MEDLINE | ID: mdl-26945051
ABSTRACT
In plants, clathrin-mediated endocytosis (CME) is dependent on the function of clathrin and its accessory heterooligomeric adaptor protein complexes, ADAPTOR PROTEIN2 (AP-2) and the TPLATE complex (TPC), and is negatively regulated by the hormones auxin and salicylic acid (SA). The details for how clathrin and its adaptor complexes are recruited to the plasma membrane (PM) to regulate CME, however, are poorly understood. We found that SA and the pharmacological CME inhibitor tyrphostin A23 reduce the membrane association of clathrin and AP-2, but not that of the TPC, whereas auxin solely affected clathrin membrane association, in Arabidopsis (Arabidopsis thaliana). Genetic and pharmacological experiments revealed that loss of AP2µ or AP2σ partially affected the membrane association of other AP-2 subunits and that the AP-2 subunit AP2σ, but not AP2µ, was required for SA- and tyrphostin A23-dependent inhibition of CME Furthermore, we show that although AP-2 and the TPC are both required for the PM recruitment of clathrin in wild-type cells, the TPC is necessary for clathrin PM association in AP-2-deficient cells. These results indicate that developmental signals may differentially modulate the membrane recruitment of clathrin and its core accessory complexes to regulate the process of CME in plant cells.
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Full text: 1 Database: MEDLINE Main subject: Clathrin / Arabidopsis / Arabidopsis Proteins / Adaptor Protein Complex 2 / Endocytosis / Membranes Language: En Year: 2016 Type: Article
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Full text: 1 Database: MEDLINE Main subject: Clathrin / Arabidopsis / Arabidopsis Proteins / Adaptor Protein Complex 2 / Endocytosis / Membranes Language: En Year: 2016 Type: Article