Mitochondria-Targeted Reaction-Based Fluorescent Probe for Hydrogen Sulfide.
Anal Chem
; 88(10): 5476-81, 2016 05 17.
Article
in En
| MEDLINE
| ID: mdl-27094621
ABSTRACT
In this study, we developed a turn-on mitochondria-targeting hydrogen sulfide, "probe 1", based on the selective thiolysis of 7-nitro-1,2,3-benzoxadiazole amine moiety attached to the piperazine-based naphthalimide scaffold. Probe 1 exhibited excellent properties with 68-fold fluorescence enhancement, a low detection limit (2.46 µM), a low cytotoxicity, and a good selectivity toward hydrogen sulfide. The success of intracellular imaging indicated that probe 1 could be used in further applications for the investigation of biological functions and pathological roles of H2S in living systems.
Full text:
1
Database:
MEDLINE
Main subject:
Fluorescent Dyes
/
Hydrogen Sulfide
/
Mitochondria
Limits:
Humans
Language:
En
Year:
2016
Type:
Article