An Accurate In Vitro Model of the E.â
coli Envelope.
Angew Chem Weinheim Bergstr Ger
; 127(41): 12120-12123, 2015 Oct 05.
Article
in En
| MEDLINE
| ID: mdl-27346898
ABSTRACT
Gram-negative bacteria are an increasingly serious source of antibiotic-resistant infections, partly owing to their characteristic protective envelope. This complex, 20â
nm thick barrier includes a highly impermeable, asymmetric bilayer outer membrane (OM), which plays a pivotal role in resisting antibacterial chemotherapy. Nevertheless, the OM molecular structure and its dynamics are poorly understood because the structure is difficult to recreate or study inâ
vitro. The successful formation and characterization of a fully asymmetric model envelope using Langmuir-Blodgett and Langmuir-Schaefer methods is now reported. Neutron reflectivity and isotopic labeling confirmed the expected structure and asymmetry and showed that experiments with antibacterial proteins reproduced published inâ
vivo behavior. By closely recreating natural OM behavior, this model provides a much needed robust system for antibiotic development.
Full text:
1
Database:
MEDLINE
Type of study:
Prognostic_studies
Language:
En
Year:
2015
Type:
Article