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Human odontogenic epithelial cells derived from epithelial rests of Malassez possess stem cell properties.
Tsunematsu, Takaaki; Fujiwara, Natsumi; Yoshida, Maki; Takayama, Yukihiro; Kujiraoka, Satoko; Qi, Guangying; Kitagawa, Masae; Kondo, Tomoyuki; Yamada, Akiko; Arakaki, Rieko; Miyauchi, Mutsumi; Ogawa, Ikuko; Abiko, Yoshihiro; Nikawa, Hiroki; Murakami, Shinya; Takata, Takashi; Ishimaru, Naozumi; Kudo, Yasusei.
Affiliation
  • Tsunematsu T; Department of Oral Molecular Pathology, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, Japan.
  • Fujiwara N; Department of Oral Healthcare Promotion, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, Japan.
  • Yoshida M; Department of Oral and Maxillofacial Pathobiology, Institute of Biomedical and Health Sciences, Hiroshima University Graduate School, Hiroshima, Japan.
  • Takayama Y; Department of Oral and Maxillofacial Pathobiology, Institute of Biomedical and Health Sciences, Hiroshima University Graduate School, Hiroshima, Japan.
  • Kujiraoka S; Department of Oral Biology and Engineering, Division of Oral Health Sciences, Institute of Biomedical Sciences, Hiroshima University Graduate School, Hiroshima, Japan.
  • Qi G; Department of Oral Molecular Pathology, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, Japan.
  • Kitagawa M; Department of Pathology and Physiopathology, Guilin Medical University, Guilin, China.
  • Kondo T; Center of Oral Clinical Examination, Hiroshima University Hospital, Hiroshima, Japan.
  • Yamada A; Department of Oral Molecular Pathology, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, Japan.
  • Arakaki R; Department of Oral Molecular Pathology, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, Japan.
  • Miyauchi M; Department of Oral Molecular Pathology, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, Japan.
  • Ogawa I; Department of Oral and Maxillofacial Pathobiology, Institute of Biomedical and Health Sciences, Hiroshima University Graduate School, Hiroshima, Japan.
  • Abiko Y; Center of Oral Clinical Examination, Hiroshima University Hospital, Hiroshima, Japan.
  • Nikawa H; Division of Oral Medicine and Pathology, Department of Human Biology and Pathophysiology, School of Dentistry, Health Sciences University of Hokkaido, Hokkaido, Japan.
  • Murakami S; Department of Oral Biology and Engineering, Division of Oral Health Sciences, Institute of Biomedical Sciences, Hiroshima University Graduate School, Hiroshima, Japan.
  • Takata T; Department of Periodontology, Osaka University Graduate School of Dentistry, Osaka, Japan.
  • Ishimaru N; Department of Oral and Maxillofacial Pathobiology, Institute of Biomedical and Health Sciences, Hiroshima University Graduate School, Hiroshima, Japan.
  • Kudo Y; Department of Oral Molecular Pathology, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, Japan.
Lab Invest ; 96(10): 1063-75, 2016 10.
Article in En | MEDLINE | ID: mdl-27479086
ABSTRACT
Epithelial cell rests of Malassez (ERM) are quiescent epithelial remnants of the Hertwig's epithelial root sheath (HERS) that are involved in the formation of tooth roots. ERM cells are unique epithelial cells that remain in periodontal tissues throughout adult life. They have a functional role in the repair/regeneration of cement or enamel. Here, we isolated odontogenic epithelial cells from ERM in the periodontal ligament, and the cells were spontaneously immortalized. Immortalized odontogenic epithelial (iOdE) cells had the ability to form spheroids and expressed stem cell-related genes. Interestingly, iOdE cells underwent osteogenic differentiation, as demonstrated by the mineralization activity in vitro in mineralization-inducing media and formation of calcification foci in iOdE cells transplanted into immunocompromised mice. These findings suggest that a cell population with features similar to stem cells exists in ERM and that this cell population has a differentiation capacity for producing calcifications in a particular microenvironment. In summary, iOdE cells will provide a convenient cell source for tissue engineering and experimental models to investigate tooth growth, differentiation, and tumorigenesis.
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Full text: 1 Database: MEDLINE Main subject: Periodontal Ligament / Odontogenesis Type of study: Prognostic_studies Limits: Humans Language: En Year: 2016 Type: Article
Fulltext
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PubMed Links
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Full text: 1 Database: MEDLINE Main subject: Periodontal Ligament / Odontogenesis Type of study: Prognostic_studies Limits: Humans Language: En Year: 2016 Type: Article