Discovery of LRE1 as a specific and allosteric inhibitor of soluble adenylyl cyclase.
Nat Chem Biol
; 12(10): 838-44, 2016 10.
Article
in En
| MEDLINE
| ID: mdl-27547922
ABSTRACT
The prototypical second messenger cAMP regulates a wide variety of physiological processes. It can simultaneously mediate diverse functions by acting locally in independently regulated microdomains. In mammalian cells, two types of adenylyl cyclase generate cAMP G-protein-regulated transmembrane adenylyl cyclases and bicarbonate-, calcium- and ATP-regulated soluble adenylyl cyclase (sAC). Because each type of cyclase regulates distinct microdomains, methods to distinguish between them are needed to understand cAMP signaling. We developed a mass-spectrometry-based adenylyl cyclase assay, which we used to identify a new sAC-specific inhibitor, LRE1. LRE1 bound to the bicarbonate activator binding site and inhibited sAC via a unique allosteric mechanism. LRE1 prevented sAC-dependent processes in cellular and physiological systems, and it will facilitate exploration of the therapeutic potential of sAC inhibition.
Full text:
1
Database:
MEDLINE
Main subject:
Pyrimidines
/
Thiophenes
/
Adenylyl Cyclases
/
Adenylyl Cyclase Inhibitors
Limits:
Humans
Language:
En
Year:
2016
Type:
Article