Radiation inactivation analysis of the A1 adenosine receptor of rat brain. Decrease in radiation inactivation size in the presence of guanine nucleotide.
FEBS Lett
; 252(1-2): 125-8, 1989 Jul 31.
Article
in En
| MEDLINE
| ID: mdl-2759228
ABSTRACT
Radiation inactivation analysis of the binding of the A1 adenosine receptor antagonist, 8-cyclopentyl-1,3-dipropylxanthine to rat brain membranes yielded a radiation inactivation size of 58 kDa. In the presence of GTP gamma S this was reduced to 33 kDa, in good agreement with the size of the ligand-binding subunit detected after photoaffinity labelling. The data indicate that the structural association of A1 adenosine receptors with G-protein components is altered in situ in the presence of guanine nucleotides.
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Database:
MEDLINE
Main subject:
Xanthines
/
Brain
/
Adenosine
/
Receptors, Purinergic
/
Guanine Nucleotides
Limits:
Animals
Language:
En
Year:
1989
Type:
Article