DiAna, an ImageJ tool for object-based 3D co-localization and distance analysis.
Methods
; 115: 55-64, 2017 02 15.
Article
in En
| MEDLINE
| ID: mdl-27890650
ABSTRACT
We present a new plugin for ImageJ called DiAna, for Distance Analysis, which comes with a user-friendly interface. DiAna proposes robust and accurate 3D segmentation for object extraction. The plugin performs automated object-based co-localization and distance analysis. DiAna offers an in-depth analysis of co-localization between objects and retrieves 3D measurements including co-localizing volumes and surfaces of contact. It also computes the distribution of distances between objects in 3D. With DiAna, we furthermore introduce an original method, which allows for estimating the statistical significance of object co-localization. DiAna offers a complete and intuitive 3D image analysis tool for biologists.
Key words
Full text:
1
Database:
MEDLINE
Main subject:
Image Processing, Computer-Assisted
/
Software
/
Brain
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Microscopy, Confocal
/
Microscopy, Fluorescence
Limits:
Animals
Language:
En
Year:
2017
Type:
Article