DNA loop domain organization in nucleoids from cells of different types.
Biochem Biophys Res Commun
; 483(1): 142-146, 2017 01 29.
Article
in En
| MEDLINE
| ID: mdl-28039056
ABSTRACT
The loop domain organization of chromatin plays an important role in transcription regulation and thus may be assumed to vary in cells of different types. We investigated the kinetics of DNA loop migration during single cell gel electrophoresis (the comet assay) for nucleoids obtained from human lymphocytes, lymphoblasts and glioblastoma T98G cells. The results confirm our previous observation that there are three parts of DNA in nucleoids DNA on the nucleoid surface, loops up to â¼150 kb inside the nucleoid, and larger loops that cannot migrate. However, the relative amounts of the three parts were found to be very different for different cell types. The distributions of the loop length up to 150 kb were shown to be exponential, with the distribution parameter, the loop density, to be dependent on the cell type.
Key words
Full text:
1
Database:
MEDLINE
Main subject:
DNA
/
Comet Assay
Limits:
Adult
/
Female
/
Humans
/
Male
Language:
En
Year:
2017
Type:
Article