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Mediator, SWI/SNF and SAGA complexes regulate Yap8-dependent transcriptional activation of ACR2 in response to arsenate.
Menezes, Regina Andrade; Pimentel, Catarina; Silva, Ana Rita Courelas; Amaral, Catarina; Merhej, Jawad; Devaux, Frédéric; Rodrigues-Pousada, Claudina.
Affiliation
  • Menezes RA; Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Oeiras, Portugal.
  • Pimentel C; Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Oeiras, Portugal.
  • Silva AR; Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Oeiras, Portugal.
  • Amaral C; Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Oeiras, Portugal.
  • Merhej J; Sorbonne Universités, UPMC Univ Paris 06, UMR 7238, Laboratoire de Génomique des Microorganismes, F-75005 Paris, France; CNRS, UMR 7238, Laboratoire de Génomique des Microorganismes, F-75005 Paris, France.
  • Devaux F; Sorbonne Universités, UPMC Univ Paris 06, UMR 7238, Laboratoire de Génomique des Microorganismes, F-75005 Paris, France; CNRS, UMR 7238, Laboratoire de Génomique des Microorganismes, F-75005 Paris, France.
  • Rodrigues-Pousada C; Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Oeiras, Portugal. Electronic address: claudina@itqb.unl.pt.
Biochim Biophys Acta Gene Regul Mech ; 1860(4): 472-481, 2017 Apr.
Article in En | MEDLINE | ID: mdl-28188921
ABSTRACT
Response to arsenic stress in Saccharomyces cerevisiae is orchestrated by the regulatory protein Yap8, which mediates transcriptional activation of ACR2 and ACR3. This study contributes to the state of art knowledge of the molecular mechanisms underlying yeast stress response to arsenate as it provides the genetic and biochemical evidences that Yap8, through cysteine residues 132, 137, and 274, is the sensor of presence of arsenate in the cytosol. Moreover, it is here reported for the first time the essential role of the Mediator complex in the transcriptional activation of ACR2 by Yap8. Based on our data, we propose an order-of-function map to recapitulate the sequence of events taking place in cells injured with arsenate. Modification of the sulfhydryl state of these cysteines converts Yap8 in its activated form, triggering the recruitment of the Mediator complex to the ACR2/ACR3 promoter, through the interaction with the tail subunit Med2. The Mediator complex then transfers the regulatory signals conveyed by Yap8 to the core transcriptional machinery, which culminates with TBP occupancy, ACR2 upregulation and cell adaptation to arsenate stress. Additional co-factors are required for the transcriptional activation of ACR2 by Yap8, particularly the nucleosome remodeling activity of SWI/SNF and SAGA complexes.
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Full text: 1 Database: MEDLINE Main subject: Arsenates / Saccharomyces cerevisiae / Transcriptional Activation / Saccharomyces cerevisiae Proteins / Multiprotein Complexes / Basic-Leucine Zipper Transcription Factors / Arsenate Reductases / Mediator Complex Language: En Year: 2017 Type: Article

Full text: 1 Database: MEDLINE Main subject: Arsenates / Saccharomyces cerevisiae / Transcriptional Activation / Saccharomyces cerevisiae Proteins / Multiprotein Complexes / Basic-Leucine Zipper Transcription Factors / Arsenate Reductases / Mediator Complex Language: En Year: 2017 Type: Article