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Atomic-resolution structures from fragmented protein crystals with the cryoEM method MicroED.
de la Cruz, M Jason; Hattne, Johan; Shi, Dan; Seidler, Paul; Rodriguez, Jose; Reyes, Francis E; Sawaya, Michael R; Cascio, Duilio; Weiss, Simon C; Kim, Sun Kyung; Hinck, Cynthia S; Hinck, Andrew P; Calero, Guillermo; Eisenberg, David; Gonen, Tamir.
Affiliation
  • de la Cruz MJ; Howard Hughes Medical Institute, Janelia Research Campus, Ashburn, Virginia, USA.
  • Hattne J; Howard Hughes Medical Institute, Janelia Research Campus, Ashburn, Virginia, USA.
  • Shi D; Howard Hughes Medical Institute, Janelia Research Campus, Ashburn, Virginia, USA.
  • Seidler P; Howard Hughes Medical Institute, UCLA-DOE Institute, Departments of Biological Chemistry, Chemistry &Biochemistry, and Molecular Biology Institute, UCLA, Los Angeles, California, USA.
  • Rodriguez J; Howard Hughes Medical Institute, UCLA-DOE Institute, Departments of Biological Chemistry, Chemistry &Biochemistry, and Molecular Biology Institute, UCLA, Los Angeles, California, USA.
  • Reyes FE; Howard Hughes Medical Institute, Janelia Research Campus, Ashburn, Virginia, USA.
  • Sawaya MR; Howard Hughes Medical Institute, UCLA-DOE Institute, Departments of Biological Chemistry, Chemistry &Biochemistry, and Molecular Biology Institute, UCLA, Los Angeles, California, USA.
  • Cascio D; Howard Hughes Medical Institute, UCLA-DOE Institute, Departments of Biological Chemistry, Chemistry &Biochemistry, and Molecular Biology Institute, UCLA, Los Angeles, California, USA.
  • Weiss SC; Department of Structural Biology, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.
  • Kim SK; Department of Structural Biology, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.
  • Hinck CS; Department of Structural Biology, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.
  • Hinck AP; Department of Structural Biology, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.
  • Calero G; Department of Structural Biology, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.
  • Eisenberg D; Howard Hughes Medical Institute, UCLA-DOE Institute, Departments of Biological Chemistry, Chemistry &Biochemistry, and Molecular Biology Institute, UCLA, Los Angeles, California, USA.
  • Gonen T; Howard Hughes Medical Institute, Janelia Research Campus, Ashburn, Virginia, USA.
Nat Methods ; 14(4): 399-402, 2017 Feb 13.
Article in En | MEDLINE | ID: mdl-28192420
ABSTRACT
Traditionally, crystallographic analysis of macromolecules has depended on large, well-ordered crystals, which often require significant effort to obtain. Even sizable crystals sometimes suffer from pathologies that render them inappropriate for high-resolution structure determination. Here we show that fragmentation of large, imperfect crystals into microcrystals or nanocrystals can provide a simple path for high-resolution structure determination by the cryoEM method MicroED and potentially by serial femtosecond crystallography.
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Full text: 1 Database: MEDLINE Main subject: Proteins / Cryoelectron Microscopy / Crystallography Language: En Year: 2017 Type: Article
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PubMed Links
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Full text: 1 Database: MEDLINE Main subject: Proteins / Cryoelectron Microscopy / Crystallography Language: En Year: 2017 Type: Article