RIPK3 Restricts Viral Pathogenesis via Cell Death-Independent Neuroinflammation.
Cell
; 169(2): 301-313.e11, 2017 Apr 06.
Article
in En
| MEDLINE
| ID: mdl-28366204
ABSTRACT
Receptor-interacting protein kinase-3 (RIPK3) is an activator of necroptotic cell death, but recent work has implicated additional roles for RIPK3 in inflammatory signaling independent of cell death. However, while necroptosis has been shown to contribute to antiviral immunity, death-independent roles for RIPK3 in host defense have not been demonstrated. Using a mouse model of West Nile virus (WNV) encephalitis, we show that RIPK3 restricts WNV pathogenesis independently of cell death. Ripk3-/- mice exhibited enhanced mortality compared to wild-type (WT) controls, while mice lacking the necroptotic effector MLKL, or both MLKL and caspase-8, were unaffected. The enhanced susceptibility of Ripk3-/- mice arose from suppressed neuronal chemokine expression and decreased central nervous system (CNS) recruitment of T lymphocytes and inflammatory myeloid cells, while peripheral immunity remained intact. These data identify pleiotropic functions for RIPK3 in the restriction of viral pathogenesis and implicate RIPK3 as a key coordinator of immune responses within the CNS.
Key words
Full text:
1
Database:
MEDLINE
Main subject:
West Nile Fever
/
West Nile virus
/
Receptor-Interacting Protein Serine-Threonine Kinases
Type of study:
Etiology_studies
Limits:
Animals
Language:
En
Year:
2017
Type:
Article