Creating cell type-specific mutants by enhancer mutagenesis.
Genes Dev
; 31(7): 629-631, 2017 04 01.
Article
in En
| MEDLINE
| ID: mdl-28446593
ABSTRACT
Cell signaling plays an essential role in development, and knowledge of the identities of the cells sending the signal is critical. This can be a challenge, since signaling pathways and ligands are commonly used at multiple times and in multiple cell types during development. One solution to this problem is to create cell type-specific mutants using CRISPR/Cas9 to mutate enhancers that control different patterns of expression. In this issue of Genes & Development, Rogers and colleagues (pp. 634-638) provide the first use of this method in Drosophila to solve a long-standing issue in patterning of the embryonic central nervous system.
Key words
Full text:
1
Database:
MEDLINE
Main subject:
Gene Expression Regulation, Developmental
/
Drosophila Proteins
Limits:
Animals
Language:
En
Year:
2017
Type:
Article