The amino acid sequence of pancreatic spasmolytic polypeptide.
Biochim Biophys Acta
; 827(3): 410-8, 1985 Mar 01.
Article
in En
| MEDLINE
| ID: mdl-2857575
ABSTRACT
The sequence of porcine pancreatic spasmolytic polypeptide has been established by a variety of techniques including manual as well as automatic sequencing of fragments resulting from the cleavage of reduced and S-carboxymethylated pancreatic spasmolytic polypeptide with trypsin, chymotrypsin, clostripain, cyanogen bromide and formic acid. The N- and C-terminal sequences were established using pyroglutamate amino-peptidase and carboxypeptidase A, respectively. Pancreatic spasmolytic polypeptide contains 106 amino acid residues in a single chain with seven S-S bridges and a pyroglutamyl blocked N-terminal. The alignment of the sequences representing amino acids 14-49 and 63-98 shows pair-wise identical amino acid residues in 18 out of 36 positions, indicating that these two "domains" have been derived from a common gene.
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Database:
MEDLINE
Main subject:
Peptides
/
Neuropeptides
/
Cysteine Endopeptidases
/
Mucins
/
Muscle Proteins
Limits:
Animals
Language:
En
Year:
1985
Type:
Article