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Three-phase partitioning as an elegant and versatile platform applied to nonchromatographic bioseparation processes.
Yan, Jing-Kun; Wang, Yao-Yao; Qiu, Wen-Yi; Ma, Haile; Wang, Zhen-Bin; Wu, Jian-Yong.
Affiliation
  • Yan JK; a Jiangsu University , Zhenjiang , China.
  • Wang YY; a Jiangsu University , Zhenjiang , China.
  • Qiu WY; a Jiangsu University , Zhenjiang , China.
  • Ma H; a Jiangsu University , Zhenjiang , China.
  • Wang ZB; a Jiangsu University , Zhenjiang , China.
  • Wu JY; b The Hong Kong Polytechnic University, Department of Applied Biology & Chemical Technology , State Key Laboratory of Chinese Medicine and Molecular Pharmacology in Shenzhen , Hung Hom, Kowloon, Hong Kong.
Crit Rev Food Sci Nutr ; 58(14): 2416-2431, 2018.
Article in En | MEDLINE | ID: mdl-28609145
In recent years, a big trend has been the development of rapid, green, efficient, economical, and scalable approaches for the separation and purification of bioactive molecules from natural sources, which can be used in food, cosmetics, and medicine. As a new nonchromatographic bioseparation technology, three-phase partitioning (TPP) is attracting the attention of a growing number of scientists and engineers. Although a number of studies have been published in the last 40 years regarding the extraction, separation, and purification of numerous bioactive molecules using TPP systems, a background review on TPP partitioning fundamentals and its applications is much needed. Therefore, the present review focuses in detail on the TPP separation process, including the definition of TPP, partitioning mechanisms, parameters for establishing the suitable condition to form precipitate such as concentration of ammonium sulfate, content of tert-butanol, pH and temperature, and the application for separation and purification of protein, enzyme, plant oil, polysaccharide, and other small molecule organic compounds. In addition, the possible directions of future developments in TPP technology are discussed. The review presents a good opportunity, as well as a challenge for scientists, to understand the detailed partitioning rule and to take better use of TPP for the production and separation of various bioactive molecules, which have been intensively applied in the food and medical fields.
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Full text: 1 Database: MEDLINE Main subject: Functional Food / Chemical Fractionation Language: En Year: 2018 Type: Article

Full text: 1 Database: MEDLINE Main subject: Functional Food / Chemical Fractionation Language: En Year: 2018 Type: Article