Participation of osteoclastogenic factors in immunopathogenesis of human chronic periapical lesions.
J Oral Pathol Med
; 46(9): 846-852, 2017 Oct.
Article
in En
| MEDLINE
| ID: mdl-28731540
ABSTRACT
BACKGROUND:
Chronic periapical lesions (CPLs) are common lesions of the oral cavity and are the result of caries, tooth fracture, iatrogenic causes, or factors causing contamination and pulp necrosis. Inflammatory cells participate in the expansion of CPLs by releasing factors that stimulate or inhibit osteolytic activity. The objective of this study was to investigate the participation of RANKL, TNF-α, cathepsin K, IL-33, and OPG in the development of radicular cysts (RCs) and periapical granulomas (PGs).METHODS:
Paraffin-embedded sections of 30 RCs and 22 PGs were submitted to immunohistochemistry.RESULTS:
Immunoexpression of the proteins studied was observed in the epithelium and capsule of RCs, as well as in connective tissue of PGs. The expression of the osteoclastogenic factors studied differed significantly in RCs and PGs (P < .001), with lower expression of OPG in RCs. In PGs, the lowest expression was observed for cathepsin K. Comparison of the 2 lesions showed a similar participation of RANKL and IL33, while a significant difference was observed for OPG (P < .001), TNF-α (P = .002), and cathepsin K (P = .016). No association of the expression of the proteins with lesions size was observed.CONCLUSIONS:
This study demonstrated the participation of RANKL, TNF-α, IL-33, cathepsin K, and OPG in the development of RCs and PGs, with emphasis on the highest immunoreactivity of cathepsin in RCs and TNF-α and OPG in PGs. OPG possibly determines the slower growth of PGs compared to RCs.Key words
Full text:
1
Database:
MEDLINE
Main subject:
Osteogenesis
/
Periapical Granuloma
/
Radicular Cyst
Limits:
Adult
/
Female
/
Humans
/
Male
Language:
En
Year:
2017
Type:
Article