Spiribacter aquaticus sp. nov., a novel member of the genus Spiribacter isolated from a saltern.
Int J Syst Evol Microbiol
; 67(8): 2947-2952, 2017 Aug.
Article
in En
| MEDLINE
| ID: mdl-28820120
ABSTRACT
A moderately halophilic bacterium, designated strain SP30T, was isolated from a solar saltern located in Santa Pola, Alicante, on the East coast of Spain. It was a Gram-stain-negative, strictly aerobic bacterium, able to grow in 7.5-25â% (w/v) NaCl and optimally in 12.5â% (w/v) NaCl. Phylogenetic analyses, based on 16S rRNA gene sequences, showed that the novel isolate is a member of the genus Spiribacter, with the most closely related species being Spiribacter roseus SSL50T (99.9â% sequence similarity) and Spiribacter curvatus UAH-SP71T (99.4â% sequence similarity). The 16S rRNA gene sequence similarity with the type species Spiribacter salinus M19-40T was 96.6â%. The DNA-DNA relatedness value between strain SP30T and S. roseus SSL50T and S. curvatus UAH-SP71T was 40 and 55â%, respectively; these values are lower than the 70â% threshold accepted for species delineation. The major fatty acids were C160, C18ââ1ω7c, C19ââ0 cyclo ω8c and C12ââ0. Similarly to other species of the genus Spiribacter, strain SP30Twas observed as curved rods and spiral cells. Metabolic versatility was reduced to the utilization of a few organic compounds as the sole carbon and energy sources, as with other members of Spiribacter. However, it differed in terms of colony pigmentation (brownish-yellow instead of pink) and in having a higher growth rate. Based on these data and on the phenotypic, genotypic and chemotaxonomic characterization, we propose the classification of strain SP30T as a novel species within the genus Spiribacter, with the name Spiribacter aquaticus sp. nov. The type strain is SP30T (=CECT 9238T=LMG 30005T).
Full text:
1
Database:
MEDLINE
Main subject:
Phylogeny
/
Ectothiorhodospiraceae
/
Salinity
Country/Region as subject:
Europa
Language:
En
Year:
2017
Type:
Article