Anti-CD25 monoclonal antibody enhances the protective efficacy of Schistosoma japonicum GST vaccine via inhibition of CD4+CD25+Foxp3+ regulatory T cells.
Parasitol Res
; 116(10): 2727-2732, 2017 Oct.
Article
in En
| MEDLINE
| ID: mdl-28825137
ABSTRACT
The effect of anti-CD25 monoclonal antibody (anti-CD25 mAb) on the protection efficacy of Schistosoma japonicum 26 kDa GST (glutathione-S-transferase) vaccine was evaluated. Mice were immunized with GST before infection with S. japonicum cercariae and then injected with anti-CD25 mAb. The worm reduction rate was promoted from 24.18% in mice with GST immunization to 47.09% in mice with GST plus anti-CD25 mAb. Compared with the control group, the percentages of splenic CD4+CD25+Foxp3+ regulatory T cells (Tregs) were significantly lower after administration of anti-CD25 mAb; meanwhile, elevated levels of IFN-γ and IL-2 were secreted by splenocytes. These results indicate that the poor protective efficacy of the GST vaccine against S. japonicum results from the presence of CD4+CD25+Foxp3+ Tregs, while anti-CD25 mAb can partially block CD4+CD25+Foxp3+ Tregs and thus enhance the protective efficacy of the GST vaccine.
Key words
Full text:
1
Database:
MEDLINE
Main subject:
Schistosoma japonicum
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Schistosomiasis japonica
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Vaccines
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Helminth Proteins
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T-Lymphocytes, Regulatory
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Glutathione Transferase
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Antibodies, Monoclonal
Limits:
Animals
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Female
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Humans
Language:
En
Year:
2017
Type:
Article