Comparative study of esterases in deltamethrin and diazinon resistant Rhipicephalus microplus and Hyalomma anatolicum ticks collected from the Trans-Gangetic plains of India.
Exp Appl Acarol
; 73(1): 115-127, 2017 Sep.
Article
in En
| MEDLINE
| ID: mdl-28894981
ABSTRACT
A comparative analysis of esterases in susceptible and resistant ticks revealed six types of esterases (EST-1b, EST-2b, EST-3b, EST-4b, EST-5b and EST-6b) in Rhipicephalus microplus and four types (EST-1h, EST-2h, EST-3h, EST-4h) in Hyalomma anatolicum using α-naphthyl acetate substrate. Inhibition studies with eserine sulfate, p-chloromercuribenzoate, copper sulphate and phenylmethylsulfonyl fluoride revealed a marked variation in band intensity between susceptible and resistant ticks, with the latter being more intense. Qualitative expression of EST-4b along with an extra band of EST-5b and EST-6b were indicative of deltamethrin and diazinon resistance in R. microplus, whereas qualitative expression of EST-4h was probably responsible for diazinon resistance in H. anatolicum. The data suggest that increased esterase activity may represent a detoxification strategy leading to the development of resistance in these tick populations.
Key words
Full text:
1
Database:
MEDLINE
Main subject:
Pyrethrins
/
Drug Resistance
/
Ixodidae
/
Diazinon
/
Esterases
/
Acaricides
/
Arthropod Proteins
/
Nitriles
Type of study:
Qualitative_research
Limits:
Animals
Country/Region as subject:
Asia
Language:
En
Year:
2017
Type:
Article