Piwi Is Required to Limit Exhaustion of Aging Somatic Stem Cells.
Cell Rep
; 20(11): 2527-2537, 2017 Sep 12.
Article
in En
| MEDLINE
| ID: mdl-28903034
ABSTRACT
Sophisticated mechanisms that preserve genome integrity are critical to ensure the maintenance of regenerative capacity while preventing transformation of somatic stem cells (SCs), yet little is known about mechanisms regulating genome maintenance in these cells. Here, we show that intestinal stem cells (ISCs) induce the Argonaute family protein Piwi in response to JAK/STAT signaling during acute proliferative episodes. Piwi function is critical to ensure heterochromatin maintenance, suppress retrotransposon activation, and prevent DNA damage in homeostasis and under regenerative pressure. Accordingly, loss of Piwi results in the loss of actively dividing ISCs and their progenies by apoptosis. We further show that Piwi expression is sufficient to allay age-related retrotransposon expression, DNA damage, apoptosis, and mis-differentiation phenotypes in the ISC lineage, improving epithelial homeostasis. Our data identify a role for Piwi in the regulation of somatic SC function, and they highlight the importance of retrotransposon control in somatic SC maintenance.
Key words
Full text:
1
Database:
MEDLINE
Main subject:
Cellular Senescence
/
Drosophila Proteins
/
Drosophila melanogaster
/
Adult Stem Cells
/
Argonaute Proteins
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Year:
2017
Type:
Article