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The Sox2 promoter-driven CD63-GFP transgenic rat model allows tracking of neural stem cell-derived extracellular vesicles.
Yoshimura, Aya; Adachi, Naoki; Matsuno, Hitomi; Kawamata, Masaki; Yoshioka, Yusuke; Kikuchi, Hisae; Odaka, Haruki; Numakawa, Tadahiro; Kunugi, Hiroshi; Ochiya, Takahiro; Tamai, Yoshitaka.
Affiliation
  • Yoshimura A; Division of Laboratory Animals Resources, National Institute of Neuroscience, National Center of Neurology and Psychiatry (NCNP), 4-1-1 Ogawa-Higashi, Kodaira, Tokyo 187-8502, Japan.
  • Adachi N; Department of Mental Disorder Research, National Institute of Neuroscience, NCNP, 4-1-1 Ogawa-Higashi, Kodaira, Tokyo 187-8502, Japan.
  • Matsuno H; Education and Research Facility of Animal Models for Human Diseases, Center for Research Promotion and Support, Fujita Health University, 1-98 Dengakugakubo, Kutsukake-cho, Toyoake, Aichi 470-1192, Japan.
  • Kawamata M; Department of Mental Disorder Research, National Institute of Neuroscience, NCNP, 4-1-1 Ogawa-Higashi, Kodaira, Tokyo 187-8502, Japan.
  • Yoshioka Y; Department of Biomedical Chemistry, School of Science and Technology, Kwansei Gakuin University, 2-1 Gakuen, Sanda, Hyogo 669-1337, Japan.
  • Kikuchi H; Department of Mental Disorder Research, National Institute of Neuroscience, NCNP, 4-1-1 Ogawa-Higashi, Kodaira, Tokyo 187-8502, Japan.
  • Odaka H; Division of Molecular and Cellular Medicine, National Cancer Center Research Institute (NCC), 1-1 Tsukiji 5-chome, Chuo-ku, Tokyo 104-0045, Japan.
  • Numakawa T; Division of Molecular and Cellular Medicine, National Cancer Center Research Institute (NCC), 1-1 Tsukiji 5-chome, Chuo-ku, Tokyo 104-0045, Japan.
  • Kunugi H; Department of Degenerative Neurological Diseases, National Institute of Neuroscience, NCNP, 4-1-1 Ogawa-Higashi, Kodaira, Tokyo 187-8502, Japan.
  • Ochiya T; Department of Mental Disorder Research, National Institute of Neuroscience, NCNP, 4-1-1 Ogawa-Higashi, Kodaira, Tokyo 187-8502, Japan.
  • Tamai Y; Department of Life Science and Medical Bioscience, School of Advanced Science and Engineering, Waseda University, 3-4-1 Okubo, Shinjuku-ku, Tokyo 169-8555, Japan.
Dis Model Mech ; 11(1)2018 01 30.
Article in En | MEDLINE | ID: mdl-29208635
ABSTRACT
Extracellular vesicles (EVs) can modulate microenvironments by transferring biomolecules, including RNAs and proteins derived from releasing cells, to target cells. To understand the molecular mechanisms maintaining the neural stem cell (NSC) niche through EVs, a new transgenic (Tg) rat strain that can release human CD63-GFP-expressing EVs from the NSCs was established. Human CD63-GFP expression was controlled under the rat Sox2 promoter (Sox2/human CD63-GFP), and it was expressed in undifferentiated fetal brains. GFP signals were specifically observed in in vitro cultured NSCs obtained from embryonic brains of the Tg rats. We also demonstrated that embryonic NSC (eNSC)-derived EVs were labelled by human CD63-GFP. Furthermore, when we examined the transfer of EVs, eNSC-derived EVs were found to be incorporated into astrocytes and eNSCs, thus implying an EV-mediated communication between different cell types around NSCs. This new Sox2/human CD63-GFP Tg rat strain should provide resources to analyse the cell-to-cell communication via EVs in NSC microenvironments.
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Full text: 1 Database: MEDLINE Main subject: Promoter Regions, Genetic / Green Fluorescent Proteins / SOXB1 Transcription Factors / Neural Stem Cells / Tetraspanin 30 / Extracellular Vesicles Type of study: Prognostic_studies Limits: Animals / Humans Language: En Year: 2018 Type: Article
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Full text: 1 Database: MEDLINE Main subject: Promoter Regions, Genetic / Green Fluorescent Proteins / SOXB1 Transcription Factors / Neural Stem Cells / Tetraspanin 30 / Extracellular Vesicles Type of study: Prognostic_studies Limits: Animals / Humans Language: En Year: 2018 Type: Article