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CCR5 chemokine receptor gene polymorphisms in ocular toxoplasmosis.
de Faria Junior, Geraldo M; Ayo, Christiane M; de Oliveira, Amanda P; Lopes, Alessandro G; Frederico, Fábio B; Silveira-Carvalho, Aparecida P; Previato, Mariana; Barbosa, Amanda P; Murata, Fernando H A; de Almeida Junior, Gildásio Castello; Siqueira, Rubens Camargo; de Mattos, Luiz C; Brandão de Mattos, Cinara C.
Affiliation
  • de Faria Junior GM; Immunogenetics Laboratory, Molecular Biology Department, Faculdade de Medicina de São José do Rio Preto (FAMERP), São José do Rio Preto, SP, Brazil; Toxoplasma Research Group, Faculdade de Medicina de São José do Rio Preto (FAMERP), São José do Rio Preto, SP, Brazil.
  • Ayo CM; Immunogenetics Laboratory, Molecular Biology Department, Faculdade de Medicina de São José do Rio Preto (FAMERP), São José do Rio Preto, SP, Brazil; Toxoplasma Research Group, Faculdade de Medicina de São José do Rio Preto (FAMERP), São José do Rio Preto, SP, Brazil.
  • de Oliveira AP; Immunogenetics Laboratory, Molecular Biology Department, Faculdade de Medicina de São José do Rio Preto (FAMERP), São José do Rio Preto, SP, Brazil.
  • Lopes AG; Biology Department, Instituto de Biociências, Letras e Ciências Exatas (IBILCE), Universidade Estadual Paulista "Júlio de Mesquita Filho" (UNESP), São José do Rio Preto, SP, Brazil.
  • Frederico FB; Immunogenetics Laboratory, Molecular Biology Department, Faculdade de Medicina de São José do Rio Preto (FAMERP), São José do Rio Preto, SP, Brazil; Ophthalmology Outpatient Clinic of Hospital de Base da Fundação Faculdade Regional de Medicina de São José do Rio Preto (HB- FUNFARME), São José do Rio
  • Silveira-Carvalho AP; Immunogenetics Laboratory, Molecular Biology Department, Faculdade de Medicina de São José do Rio Preto (FAMERP), São José do Rio Preto, SP, Brazil.
  • Previato M; Immunogenetics Laboratory, Molecular Biology Department, Faculdade de Medicina de São José do Rio Preto (FAMERP), São José do Rio Preto, SP, Brazil; Toxoplasma Research Group, Faculdade de Medicina de São José do Rio Preto (FAMERP), São José do Rio Preto, SP, Brazil.
  • Barbosa AP; Ophthalmology Outpatient Clinic of Hospital de Base da Fundação Faculdade Regional de Medicina de São José do Rio Preto (HB- FUNFARME), São José do Rio Preto, SP, Brazil; Toxoplasma Research Group, Faculdade de Medicina de São José do Rio Preto (FAMERP), São José do Rio Preto, SP, Brazil.
  • Murata FHA; Immunogenetics Laboratory, Molecular Biology Department, Faculdade de Medicina de São José do Rio Preto (FAMERP), São José do Rio Preto, SP, Brazil; Toxoplasma Research Group, Faculdade de Medicina de São José do Rio Preto (FAMERP), São José do Rio Preto, SP, Brazil.
  • de Almeida Junior GC; Ophthalmology Outpatient Clinic of Hospital de Base da Fundação Faculdade Regional de Medicina de São José do Rio Preto (HB- FUNFARME), São José do Rio Preto, SP, Brazil; Toxoplasma Research Group, Faculdade de Medicina de São José do Rio Preto (FAMERP), São José do Rio Preto, SP, Brazil.
  • Siqueira RC; Toxoplasma Research Group, Faculdade de Medicina de São José do Rio Preto (FAMERP), São José do Rio Preto, SP, Brazil.
  • de Mattos LC; Immunogenetics Laboratory, Molecular Biology Department, Faculdade de Medicina de São José do Rio Preto (FAMERP), São José do Rio Preto, SP, Brazil; Toxoplasma Research Group, Faculdade de Medicina de São José do Rio Preto (FAMERP), São José do Rio Preto, SP, Brazil.
  • Brandão de Mattos CC; Immunogenetics Laboratory, Molecular Biology Department, Faculdade de Medicina de São José do Rio Preto (FAMERP), São José do Rio Preto, SP, Brazil; Toxoplasma Research Group, Faculdade de Medicina de São José do Rio Preto (FAMERP), São José do Rio Preto, SP, Brazil. Electronic address: Cinara.Brand
Acta Trop ; 178: 276-280, 2018 Feb.
Article in En | MEDLINE | ID: mdl-29221851
ABSTRACT
CC chemokine receptor type 5 (CCR5) is a chemokine receptor that influences the immune response to infectious and parasitic diseases. This study aimed to determine whether the CCR5Δ32 and CCR5 59029 A/G polymorphisms are associated with the development of ocular toxoplasmosis in humans. Patients with positive serology for Toxoplasma gondii were analyzed and grouped as 'with ocular toxoplasmosis' (G1 n=160) or 'without ocular toxoplasmosis' (G2 n=160). A control group (G3) consisted of 160 individuals with negative serology. The characterization of the CCR5Δ32 and CCR5 59029 A/G polymorphisms was by PCR and by PCR-RFLP, respectively. The difference between the groups with respect to the mean age (G1 mean age 47.3, SD±19.3, median 46 [range 18-95]; G2 mean age 61.3, SD±13.7, median 61 [range 21-87]; G3 mean age 38.8, SD±17.9, median 34 [range 18-80]) was statistically significant (G1 vs.G2 p-value <0.0001; t=7.21; DF=318; G1 vs.G3 p-value <0.0001; t=4.32; DF=318; G2 vs. G3 p-value <0.0001; t=9.62; DF=318). The Nagelkerke r2 value was 0.040. There were statistically significant differences for the CCR5/CCR5 (p-value=0.008; OR=0.261), AA (p-value=0.007; OR=2.974) and AG genotypes (p-value=0.018; OR=2.447) between G1 and G2. Individuals with the CCR5/CCR5 genotype and simultaneously the CCR5-59029 AA or AG genotypes have a greater risk of developing ocular toxoplasmosis (4% greater), which may be associated with a strong and persistent inflammatory response in ocular tissue.
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Full text: 1 Database: MEDLINE Main subject: Polymorphism, Genetic / Toxoplasmosis, Ocular / Receptors, CCR5 / Genetic Predisposition to Disease Type of study: Etiology_studies / Observational_studies / Risk_factors_studies Limits: Adolescent / Adult / Aged / Aged80 / Female / Humans / Male / Middle aged Language: En Year: 2018 Type: Article
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Full text: 1 Database: MEDLINE Main subject: Polymorphism, Genetic / Toxoplasmosis, Ocular / Receptors, CCR5 / Genetic Predisposition to Disease Type of study: Etiology_studies / Observational_studies / Risk_factors_studies Limits: Adolescent / Adult / Aged / Aged80 / Female / Humans / Male / Middle aged Language: En Year: 2018 Type: Article