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Global unleashing of transcription elongation waves in response to genotoxic stress restricts somatic mutation rate.
Lavigne, Matthieu D; Konstantopoulos, Dimitris; Ntakou-Zamplara, Katerina Z; Liakos, Anastasios; Fousteri, Maria.
Affiliation
  • Lavigne MD; Biomedical Sciences Research Center 'Alexander Fleming', 34 Fleming Street, Vari, 16672, Athens, Greece.
  • Konstantopoulos D; Biomedical Sciences Research Center 'Alexander Fleming', 34 Fleming Street, Vari, 16672, Athens, Greece.
  • Ntakou-Zamplara KZ; Biomedical Sciences Research Center 'Alexander Fleming', 34 Fleming Street, Vari, 16672, Athens, Greece.
  • Liakos A; Biomedical Sciences Research Center 'Alexander Fleming', 34 Fleming Street, Vari, 16672, Athens, Greece.
  • Fousteri M; Biomedical Sciences Research Center 'Alexander Fleming', 34 Fleming Street, Vari, 16672, Athens, Greece. fousteri@fleming.gr.
Nat Commun ; 8(1): 2076, 2017 12 12.
Article in En | MEDLINE | ID: mdl-29233992
ABSTRACT
Complex molecular responses preserve gene expression accuracy and genome integrity in the face of environmental perturbations. Here we report that, in response to UV irradiation, RNA polymerase II (RNAPII) molecules are dynamically and synchronously released from promoter-proximal regions into elongation to promote uniform and accelerated surveillance of the whole transcribed genome. The maximised influx of de novo released RNAPII correlates with increased damage-sensing, as confirmed by RNAPII progressive accumulation at dipyrimidine sites and by the average slow-down of elongation rates in gene bodies. In turn, this transcription elongation 'safe' mode guarantees efficient DNA repair regardless of damage location, gene size and transcription level. Accordingly, we detect low and homogenous rates of mutational signatures associated with UV exposure or cigarette smoke across all active genes. Our study reveals a novel advantage for transcription regulation at the promoter-proximal level and provides unanticipated insights into how active transcription shapes the mutagenic landscape of cancer genomes.
Subject(s)

Full text: 1 Database: MEDLINE Main subject: DNA Damage / RNA Polymerase II / Promoter Regions, Genetic / Mutation Rate / Transcription Elongation, Genetic Limits: Humans Language: En Year: 2017 Type: Article

Full text: 1 Database: MEDLINE Main subject: DNA Damage / RNA Polymerase II / Promoter Regions, Genetic / Mutation Rate / Transcription Elongation, Genetic Limits: Humans Language: En Year: 2017 Type: Article