Oncogenic Role of THOR, a Conserved Cancer/Testis Long Non-coding RNA.
Cell
; 171(7): 1559-1572.e20, 2017 Dec 14.
Article
in En
| MEDLINE
| ID: mdl-29245011
ABSTRACT
Large-scale transcriptome sequencing efforts have vastly expanded the catalog of long non-coding RNAs (lncRNAs) with varying evolutionary conservation, lineage expression, and cancer specificity. Here, we functionally characterize a novel ultraconserved lncRNA, THOR (ENSG00000226856), which exhibits expression exclusively in testis and a broad range of human cancers. THOR knockdown and overexpression in multiple cell lines and animal models alters cell or tumor growth supporting an oncogenic role. We discovered a conserved interaction of THOR with IGF2BP1 and show that THOR contributes to the mRNA stabilization activities of IGF2BP1. Notably, transgenic THOR knockout produced fertilization defects in zebrafish and also conferred a resistance to melanoma onset. Likewise, ectopic expression of human THOR in zebrafish accelerated the onset of melanoma. THOR represents a novel class of functionally important cancer/testis lncRNAs whose structure and function have undergone positive evolutionary selection.
Key words
Full text:
1
Database:
MEDLINE
Main subject:
Zebrafish
/
Disease Models, Animal
/
RNA, Long Noncoding
/
Melanoma
Limits:
Animals
/
Humans
/
Male
Language:
En
Year:
2017
Type:
Article