Your browser doesn't support javascript.
loading
Fluorescence-based immunosensor using three-dimensional CNT network structure for sensitive and reproducible detection of oral squamous cell carcinoma biomarker.
Song, Chung Kil; Oh, Eunkyul; Kang, Min Sung; Shin, Bum Seok; Han, Soo Young; Jung, Mingi; Lee, Eui Seok; Yoon, Soo-Young; Sung, Myung Mo; Ng, Wei Beng; Cho, Nam-Joon; Lee, Haiwon.
Affiliation
  • Song CK; Department of Chemistry, Hanyang University, Seoul, 04763, South Korea; Institute of Nano Science & Technology, Hanyang University, Seoul, 04763, South Korea.
  • Oh E; Department of Chemistry, Hanyang University, Seoul, 04763, South Korea.
  • Kang MS; Department of Chemistry, Hanyang University, Seoul, 04763, South Korea.
  • Shin BS; Department of Chemistry, Hanyang University, Seoul, 04763, South Korea.
  • Han SY; Department of Chemistry, Hanyang University, Seoul, 04763, South Korea.
  • Jung M; Department of Chemistry, Hanyang University, Seoul, 04763, South Korea.
  • Lee ES; Department of Oral and Maxillofacial Surgery, Korea University Guro Hospital, Seoul, 08308, South Korea.
  • Yoon SY; Department of Laboratory Medicine, Korea University College of Medicine, Seoul, 08308, South Korea.
  • Sung MM; Department of Chemistry, Hanyang University, Seoul, 04763, South Korea.
  • Ng WB; School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue, 639798, Singapore.
  • Cho NJ; School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue, 639798, Singapore.
  • Lee H; Department of Chemistry, Hanyang University, Seoul, 04763, South Korea; Institute of Nano Science & Technology, Hanyang University, Seoul, 04763, South Korea. Electronic address: haiwon@hanyang.ac.kr.
Anal Chim Acta ; 1027: 101-108, 2018 Oct 16.
Article in En | MEDLINE | ID: mdl-29866259
ABSTRACT
A hierarchical three-dimensional network of carbon nanotubes on Si pillar substrate (3DN-CNTs) was developed for the accurate detection of oral squamous cell carcinoma (OSCC) in clinical saliva samples. The 3DN-CNTs were uniformly coated with a layer of aluminum oxides to enhance structural stability during biomarker detection. Cytokeratin-19 antigen (Cyfra 21-1) was utilized as a model biomarker of OSCC for fluorescence-based immunosensor using 3DN-CNTs (3DN-CNTs sensor). The 3DN-CNTs sensor enhances the sensitivity of Cyfra 21-1 detection by increasing the density of immobilized antibody through high surface area of 3DN-CNTs and enhancing the accessibility of biomolecules through the ordered pathway of hierarchical structure. The reliable detection limit for sensing of Cyfra 21-1 was estimated as in the level of 0.5 ng/mL and the quantitative estimation of Cyfra 21-1 was analyzed by 4-parameter logistic (4-PL) model for curve-fitting analysis. Clinical applicability of 3DN-CNTs sensor was evaluated through correlation with the commercially available electrochemiluminescence (ECL) detection system in the hospital. The assay results of the two systems for clinical saliva samples showed a good linear correlation. The 3DN-CNTs sensor offers great potential for accurate diagnosis of OSCC using Cyfra 21-1 biomarker in clinical fluids.
Subject(s)
Key words
Fulltext
Print
XML
PubMed Links
Search on Google

Full text: 1 Database: MEDLINE Main subject: Mouth Neoplasms / Carcinoma, Squamous Cell / Biosensing Techniques / Biomarkers, Tumor / Keratin-19 / Antigens, Neoplasm Type of study: Diagnostic_studies / Prognostic_studies Limits: Humans Language: En Year: 2018 Type: Article
Fulltext
Print
XML
PubMed Links
Search on Google

Full text: 1 Database: MEDLINE Main subject: Mouth Neoplasms / Carcinoma, Squamous Cell / Biosensing Techniques / Biomarkers, Tumor / Keratin-19 / Antigens, Neoplasm Type of study: Diagnostic_studies / Prognostic_studies Limits: Humans Language: En Year: 2018 Type: Article