Extensive Characterization of the Human Salivary Basic Proline-Rich Protein Family by Top-Down Mass Spectrometry.
J Proteome Res
; 17(9): 3292-3307, 2018 09 07.
Article
in En
| MEDLINE
| ID: mdl-30064219
ABSTRACT
Human basic proline-rich proteins and basic glycosylated proline-rich proteins, encoded by the polymorphic PRB1-4 genes and expressed only in parotid glands, are the most complex family of adult salivary proteins. The family includes 11 parent peptides/proteins and more than 6 parent glycosylated proteins, but a high number of proteoforms with rather similar structures derive from polymorphisms and post-translational modifications. 55 new components of the family were characterized by top-down liquid chromatography-mass spectrometry and tandem-mass platforms, bringing the total number of proteoforms to 109. The new components comprise the three variants P-H S1 â A, P-Ko P36 â S, and P-Ko A41 â S and several of their naturally occurring proteolytic fragments. The paper represents an updated reference for the peptides included in the heterogeneous family of proteins encoded by PRB1/PRB4. MS data are available via ProteomeXchange with the identifier PXD009813.
Key words
Full text:
1
Database:
MEDLINE
Main subject:
Saliva
/
Protein Processing, Post-Translational
/
Salivary Proline-Rich Proteins
Limits:
Adult
/
Female
/
Humans
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Male
/
Middle aged
Language:
En
Year:
2018
Type:
Article